The PDZ and LIM domain (PDLIM) protein family is known to be linked with organ development (Krcmery et al., 2010), cytoskeletal architecture and oncogenesis (Liu et al., 2015). Little is known is the expression profile, cellular localization and function of PDLIM7 in breast cancer. In this research, we aim to generate PDLIM7 knockout (KO) cells using CRISPR-Cas9 plasmid in triple negative basal breast cancer cell line MDAMB468 by incorporating a modified enhanced green fluorescent protein plasmid (pEGFP) into the target PDLIM7 sequence and using it as a reporter of selection for successful KO cells. Upon successful knockout, we aim to investigate the effect of PDLIM7 depletion on cell migration, adhesion and expression profiles of other adhesion molecules, such as integrin, talin and kindlin. The significance of this research is that it could help us better understand the underlying function of PDLIM7 in breast cancer.
